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Species Cucumis sativus
Crop Cucumber
Pest Scientific Name Cucumber green mottle mosaic virus (CGMMV)
Additional Info
Complementary Info Synonym: Cucumber mottle virus
Type Virus
Pest Classification Is seed a pathway in this crop? Yes
Main literature references 5-3, 5-133, 5-158, 5-176
Remarks Seed is a known pathway for CGMMV in cucumber and the recommended management strategy is to evaluate seed productions by field inspection or seed testing of a representative sample of each seed lot.
Detection Is there a seed test? Yes
If yes, type of test Serological
Main literature references 5-1, 5-9, 5-73, 5-80, 5-186, 5-187
Remarks ELISA methods exist and seed tests are available in some commercial labs. The validation experiment was carried out with a limited number of seed samples. It is strongly recommended that each user validate the performance of the technique to detect the virus under specific laboratory conditions. An ELISA method is an indirect method and often positive results are confirmed using a bioassay. One reference describes a PCR method to simultaneously detect a number of viruses. However, no positive and negative plant or seed controls were included in the evaluation of the PCR technique. In addition, the positive results of the multiplex PCR were not confirmed using a technique other than PCR.
Risk mitigation Can the pest be managed by seed treatment(s)? Yes
If yes, what type(s) Physical, Chemical (seed disinfection)
Main literature references 5-12, 5-77, 5-81, 5-82, 5-186, 5-197
Remarks Heat treatment of low and medium ELISA positives has been effective in eliminating CGMMV. After heat treatment, a bioassay can be used for evaluation of the effectiveness of heat treatment. One reference reconfirmed that CGMMV cannot be eliminated from highly contaminated seed lots through heat treatment. It also verified that tri-sodium phosphate treatments were not effective on highly contaminated seed lots.

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